Development of IMBs–qPCR detection method for Yersinia enterocolitica based on the foxA gene

Yersinia enterocolitica is an important zoonotic pathogen, which seriously endangers food-safety risk. In this study, the recombinant outer membrane protein OmpF and its antibody were prepared coupled with immunomagnetic beads (IMBs) to capture Y. in food samples, combining quantitative PCR detection primers of virulence factor gene foxA for contamination. The results showed that efficiency approximately 80% using anti-OmpF antibody-immunomagnetic linearly dependent under 101–105 CFU/mL compared less than 10% other bacteria. limit 64 was obtained based on combined detect artificially contaminated milk pork samples. Compared culture method, developed IMBs–qPCR method has higher consistency, time consuming, taken together provides effective alternative rapid food.